HSF1 and neuroblastoma: To determine whether α-synuclein (α-syn, herein after) aggregation altered HSF1 expression levels, we transiently transfected SH-SY5Y neuroblastoma and HEK293 cells with GFP-tagged wild-type (WT) α-syn (GFP-α-syn WT) or GFP-tagged A53T mutant α-syn (GFP-α-syn A53T).