Oestrogen modulates HCC malignancy in vivo by reducing tumor cell invasion, arresting cell cycle progression, and promoting apoptosis, characterized by decreased expression of MMP-2, MMP-9, PCNA, cyclin A, cyclin D1, and Bcl-2, and increased expression of cleaved caspase 3. The gene discussed is CCNA2; the disease is hepatocellular carcinoma.