Multiple immunofluorescence staining for CXCR2 and various immune cell markers (CD15, neutrophils; CD68, macrophages; CD3, T cells; S100, dendritic cells) was performed (Fig. 1c), and then images were analyzed using the Vectra-Inform image analysis system to calculate the proportion of CXCR2+ cells within each cellular subset in HCC tissues. The gene discussed is CD68; the disease is hepatocellular carcinoma.