In this study, we found that miR-24 was obviously downregulated in NPC cell lines and tissue samples, and ectopic expression of miR-24 could suppress NPC cell growth and invasion through targeting FSCN1. MiR-24 functions as a novel tumor suppressor in the development and progression of NPC, proving new insight into the mechanisms of NPC carcinogenesis, and suggesting the possibility of miR-24 as a therapeutic target for NPC. The gene discussed is FSCN1; the disease is nasopharyngeal carcinoma.