AREG and osteosarcoma: To test whether AREG increased the cell migration of osteosarcoma through EGFR, we reduced the EGFR expression by transfecting EGFR siRNA (Figure 2A) and found that EGFR siRNA inhibited the AREG-induced cancer cell migration and inhibited the AREG-induced ICAM-1 upregulation of the mRNA level (Figure 2B–2C).