By western blot analysis, out of 11 human NSCLC cell lines, H522, H2405, H1869, and H1581 were negative for NLK expression, whereas H2342, A549, SPC-A-1, SW900, SK-MES-1, H1299, and H661 cells all showed positive expression of NLK at various levels (Fig. 2a). This evidence concerns the gene NLK and non-small cell lung carcinoma.