HMGB1 and ischemia: Electroacupuncture pretreatment at PC6 acupoints, but not at non-acupoints, inhibited this ischemia-induced increase in myocardial HMGB1 expression at all time points, at the level of both protein (PC6+IR vs. IR, p < 0.05, Fig. 1B–E) and mRNA (PC6+IR vs. IR, p < 0.05, Fig. 2A–D).