Moreover, ASNS inhibition significantly slowed mouse sarcoma growth in vivo only when combined with depletion of plasma asparagine, likely reflecting the ability of systemic asparagine in the tumor environment to replenish intracellular asparagine availability after ASNS inhibition. We speculate that asparagine reliance of sarcoma cells represents a metabolic vulnerability that could be exploited therapeutically to inhibit rapid tumor growth. Here, ASNS is linked to neoplasm.