KRAS and sarcoma: For asparaginase, EC50 concentrations were estimated at 0.2–0.5 IU/ml in mouse Kras;p16p19null sarcoma cells, 0.8–0.9 IU/ml in human HT1080, Rh30 and Rh41 cells and 6 IU/ml in human RD cells (Figure 4A–B).