To ascertain the most appropriate breast cancer cell line model to investigate the physiological progesterone response in the context of estrogen signalling, we assessed alterations in steady state protein levels of ERα, PR, androgen receptor (AR), Cathepsin D (CTSD) and FK506 binding protein 5 (FKBP5) in response to estrogen, progesterone and 5α-dihydrotestosterone (DHT) in a panel of breast cancer cell lines. Here, FKBP5 is linked to breast cancer.