To confirm our transcriptome analysis, we used the following criteria to select a subset of mRNAs for validation by qRT-PCR: (1) the mRNA transcripts exhibited a greater than 2-fold differential expression between control and CELF1 knockdown cells, (2) a putative GRE sequence was present in either the 5′ or 3′ UTR and (3) the mRNA transcripts encoded proteins that have significant biological role in cancer. The gene discussed is CELF1; the disease is cancer.