The results showed that restoration of E2F2, CDK3, or CDK6 significantly rescued the G1-S transition impaired by miR-214 (Figure 4F), suggesting that downregulation of E2F2, CDK3, and CDK6 is functionally important for the inhibitory effect of miR-214 on HCC cell proliferation. This evidence concerns the gene E2F2 and hepatocellular carcinoma.