Since HIF-1α gene has been reported as a transcriptional target of NF-κB [19, 20], we performed EMSA to evaluate the autophagy defect mediated transcriptional activity of NF-κB in gastric cancer cells using nuclear extracts and specific probe (containing NF-κB binding site in HIF-1α promoter). Here, NFKB1 is linked to gastric cancer.