In a human melanoma cell line panel we assessed levels and activation of IGF-1R and its effectors AKT and ERKs, and expression of O6-methylguanine-DNA methyltransferase (MGMT) that removes the most toxic TMZ-induced DNA adduct, O6-methyguanine (O6-meG; [16]; Figure 1A). The gene discussed is AKT1; the disease is melanoma.