In Fig. 5a, FCM analysis of the xenografted tumor cells showed that ALDEFLUOR-positive cells in CD117+CD44+CSC vaccine group was accounting for 0.58 % (1.68 % minus 1.10 %), and it was remarkably decreased in contrast to the SKOV3 cell vaccine group for 1.48 % (2.59 % minus 1.11 %, **p < 0.01) or non-CSC vaccine group for 1.7 % (2.77 % minus 1.07 %, **p < 0.01) or PBS group for 10.4 % (11.5 % minus 1.10 %, ***p < 0.003) as is shown in Fig. 5b. Here, CD44 is linked to neoplasm.