Because the numbers of CD117+CD44+CSCs and the ALDEFLUOR-positive cell populations that have self-renew characteristics, are closely related with the sensitivity of ovarian cancer to chemotherapy and radiotherapy as well as patients survival time [34, 35], we measured the ALDEFLUOR-positive cell changes in the vaccinated mice to analyze the CSC vaccine efficient mechanisms. This evidence concerns the gene CD44 and ovarian carcinoma.