In the present study, we determined the profile of the Wnt/β-catenin signal-related miRNA expression in ESA+CD44+CD24−lin− CSCs and ESA+CD44−CD24+lin− non-CSC tumor cells from six surgical breast cancer tissues by miRNA microarray and characterized the levels of miR-1 expression in different types of breast cancers and serum samples by quantitative RT-PCR. The gene discussed is CD44; the disease is breast cancer.