In this study, EGFR in the cytosol is analyzed by IHC staining for representing the biological significance of mitochondrial EGFR, and we found that cytosolic EGFR expression is relevant to the overall survival of NSCLC patients, but the correlation of cell surface EGFR expression and the survival is not significant (data not shown). This evidence concerns the gene EGFR and non-small cell lung carcinoma.