Moreover, the discrepancy of co-culture conditioned medium and TGF-β1 induced effects on EMT and cell viability implies that observed changes in cell viability are not caused by the acquisition of a mesenchymal phenotype (EMT), but rather an EMT associated effect of epithelial-mesenchymal crosstalk in the tumor microenvironment. Here, TGFB1 is linked to neoplasm.