Our data showed that short stimulation (15 min) with NGF could induce phosphorylation of TrkA along with activation of ERK1/2 (p42/p44 MAPK) and AKT1 kinases over the basal levels in the MM cell lines SK-MEL-28-TrkA and G-361-TrkA, previously prompted by doxycycline to express TrkA (Fig. 4). Here, AKT1 is linked to Miyoshi myopathy.