As shown in the schematic chart in Fig. 2a, Atg5flox/flox/K19CreERT+ (Atg5-deficient mice) and Atg5flox/flox mice (Ctr mice) first were treated with AOM/DSS to establish colon tumors and then injected with TAM and sacrificed. The gene discussed is ATG5; the disease is infectious otitis media.