To investigate the involvement of autophagy in colon cancer in vivo, we treated mice mutant for Atg5, an indispensable gene for autophagy [20], with azoxymethane/dextran sodium sulfate (AOM/DSS), which is an established animal model used to induce and analyze colon cancer [21, 22]. Here, ATG5 is linked to malignant colon neoplasm.