Western blotting analysis showed that TDH downregulated proteins expression in PI3K/Akt signaling pathway in transplanted tumor tissues, including PI3K, Akt, p-Akt, and NF-κB. Semiquantitative results indicate that TDH and 5 FU have significant inhibitory effect on proteins expression compared with Group NM (P < 0.05, Figure 7 and Table 4); the inhibition of protein expression in PI3K/Akt signaling pathway could be one of mechanisms of the monomer regulating EC cells growth and infiltration. This evidence concerns the gene TDH and neoplasm.