Expressions of the three isoforms of Akt and the correlation between Akt and rpS6 phosphorylation, interfered by Akt lentivirus-mediated knockdown or Akt inhibitor XII (Akti-2), were detected separately to determine the principal regulator and signaling pathway of rpS6 in NSCLC. This evidence concerns the gene RPS6 and non-small cell lung carcinoma.