Based on the weak expressions of t-rpS6 and p-rpS6 in HBE but strong in the adenocarcinoma cell line of H1650 and squamous cell carcinoma of SK-MES-1 (Fig. 3a left), along with the similarly active characteristics of H1650 and SK-MES-1 (Fig. 3a middle and Fig. 3a right), rpS6 overexpressed or knockdown plasmid was stalely transfected into HBE and H1650, SK-MES-1 individually (Fig. 3b). This evidence concerns the gene RPS6 and squamous cell carcinoma.