Expressions of the three isoforms of Akt and the correlation between Akt and rpS6 phosphorylation, interfered by Akt lentivirus-mediated knockdown or Akt inhibitor XII (Akti-2), were detected separately to determine the principal regulator and signaling pathway of rpS6 in NSCLC. The gene discussed is AKT1; the disease is non-small cell lung carcinoma.