Hematoxylin/eosin staining and AFP, CA19-9, and CEA immunohistochemical analyses were also performed on primary and PDX tumors, on MT-CHC01 cells, and on MT-CHC01 xenograft, revealing the same tissue architecture and pathological phenotype of ICC [39] (Supplementary Fig. 4). The gene discussed is AFP; the disease is intrahepatic cholangiocarcinoma.