PIK3CA and non-small cell lung carcinoma: We confirmed that IL-6 expression is down-regulated in NSCLC cells interfered for Akt1 by quantitative RT-PCR (Figure 4A, left and right panels) but is up-regulated in BEAS-2B cells expressing Akt1-E17K, PIK3CA-E545K or shPTEN (Figure 4B and Supplemental Figure S5C, respectively).