In the present study, we found that microRNA miR-744 was significantly upregulated in pancreatic cancer and enhanced a stem cell-like phenotype by inhibition of multiple negative regulators of Wnt/β-catenin pathway, including frizzled-related protein 1 (SFRP1), GSK3β, and TLE3. This evidence concerns the gene GSK3B and familial pancreatic carcinoma.