In the current study, SFRP1, GSK3β and TLE3 were directly targeted and simultaneously downregulated by miR-744 in pancreatic cancer, and the expression of SFRP1, GSK3β and TLE3 was inversely correlated with the expression of miR-744 in pancreatic cancer tissues. Here, SFRP1 is linked to familial pancreatic carcinoma.