WNT2 and colorectal carcinoma: Next, to examine the effect of Wnt2 on Wnt/β-catenin signaling in CRC, we employed the loss-of-function approaches by transducing lentiviruses encoding short-hairpin RNA (shRNA) against green fluorescence (GFP; shGFP) or Wnt2 (shWnt2) (Figure 2B), as previously performed [42, 43].