Our study suggests: (1) XNJ has an anti-autophagy effect; (2) The inhibition of autophagy via p53-DRAM signaling pathway is an important mechanism of protection by XNJ; (3) The intervention of p53-DRAM signaling by key components of pharmacological agents in this pathway may represent a targeted and mechanism-based therapeutic strategy against brain damage following stroke. Here, TP53 is linked to Stroke.