CXCR4 and HIV-1 infection: To determine whether CXCR4 gene disruption by CRISPR/Cas9 can protect Jurkat T cells from HIV-1 infection, we transduced the Jurkat T cells with the control, #6 or #7 CXCR4-gRNA/Cas9 lentivirus and performed T7EN1 assay.