The bigger size of GFP associated with TCV-GFP infections is likely due to the five extra N-terminal aa it inherited from TCV CP ORF (Fig. 5A), or the different GFP variants used (cycle 3 GFP31 in TCV-GFP versus sGFP32 in CarMV-GFP and P28-GFP). This evidence concerns the gene CP and infection.