To determine the relationship between STAT3 or NF-κB activity and colon cancer cell growth inhibitory effect of (E)-4-(3-(3,5-dimethoxyphenyl)allyl)-2-methoxyphenol, we transfected the cells with STAT3 siRNA or p50 siRNA using a transfection agent. The gene discussed is NFKB1; the disease is malignant colon neoplasm.