This conversion took place normally in PRUΔku80Δasp5 parasites and CST1 was also produced, glycosylated and targeted to the PV However, upon closer inspection of the IFA, it became obvious that cyst wall formation was already impaired just one week after induction of differentiation, and even more strikingly impaired two weeks later (Fig 8A and 8B). Here, CST1 is linked to cyst.