RUNX2 and acute myeloid leukemia: Immunoblot analysis to determine the protein expression levels of PIM1 along with PIM2 and PIM3 of AML cell lines with known FLT3-mutation status (FLT3-WT; OCI-AML3, MOLM-16, FLT3-ITD; MV4;11, MOLM-13, MOLM-14) revealed no association of PIM expression and FLT3 mutation status (Figure 1B).