EGF and glioblastoma: As shown in Figure 3A, Supplementary Figure S4A and S4B, both CrkY251 and AblY245 were immediately phosphorylated after 1min of EGF stimulation in multiple GBM lines that include U138, HS168, T98G and U118MG, suggesting CrkY251 phosphorylation is a routine post-receptor event following EGF stimulation in EGFR-expressing GBM.