Cells were treated with 35 μM of either LTX-315 or LTX-328, and a gradual increase in translocation of HMGB1 from the cell lysate to the supernatant was detected in the LTX-315-treated melanoma cells, but not in the supernatants of cells treated with the mock sequence peptide LTX-328 or a serum-free medium only (Figure 11). Here, HMGB1 is linked to melanoma.