Unfortunately we have not been successful at knocking down PIM proteins by RNA interference approaches in our MPN cell lines to further test some of our results, but previous work demonstrated a small effect on the short term growth of HEL cells upon transient knockdown of PIM1 and PIM2 [67], and more recent work showed that targeting PIMs may sensitize MPN cells to JAK2 inhibitors through down regulation of c-Myc [54]. This evidence concerns the gene JAK2 and myeloproliferative disorder.