Next, we studied the effect of Ire1α deletion on glucose-regulated gene expression, by mRNA-Seq analysis on islets after 72 h incubation in 6 mM or 18 mM glucose to chronically stimulate insulin production and because 18mM glucose was reported to cause insulin mRNA degradation by IRE1α in insulinoma cells [48]. The gene discussed is INS; the disease is pancreatic insulinoma.