Additionally, drugs currently used in cancer patients, including gemcitabine, taxol, Ara-C, doxorubicin, cisplatin, etoposide, and carboplatin, used at a dose of twice the IC50 which caused necrosis and cell death, could readily induce HMGB1 release within 24 h of treatment (Fig. 1C). Here, HMGB1 is linked to cancer.