Remarkable down-regulation of pro-inflammatory cytokines (such as TNF-α, IL-1β, COX-2 and iNOS) and up-regulation of anti-inflammatory cytokine(like IL-10) after using BMSC-EVs treatment (100 and 200μg) suggests that BMSC-EVs have an anti-inflammatory affect in TNBS-induced colitis linked with inhibition of NF-κB signaling pathways. The gene discussed is TNF; the disease is colitis.