Moreover, we separately transfected three target site blockers (TSBs), namely, AXIN2-TSB, DKK3-TSB and SFRP1-TSB, to specifically inhibit miR-582-3p binding to each of these mRNA targets in vector control and miR-582-overexpressing NSCLC cells. The gene discussed is DKK3; the disease is non-small cell lung carcinoma.