To validate the role of GRB7 in the growth of ESCC cell lines, GRB7 expression was suppressed by transient siRNA transfection in KYSE410 and TE4 cells, chosen because both harbor amplification of 17q12 and show high GRB7 mRNA expression among ESCC cell lines (data not shown). The gene discussed is GRB7; the disease is esophageal squamous cell carcinoma.