Compared to the manually detected KCs, template-detected KCs were smaller in amplitude (manual = 420 ± 219 μV vs template = 326 ± 183 μV) and had more variance in the pre-KC period (manual KCs = 1945 ± 1604 vs template-detected KCs = 7388 ± 10,708). The gene discussed is TBCE; the disease is keratoconus.