CDHR5 and autosomal dominant polycystic kidney disease: To investigate methylation-associated inactivation of the MUPCDH gene in ADPKD, serial deletion constructs for use in the luciferase assay were treated with SssI methylase in vitro, and then transfected into HEK293T cells; increased methylation of promoter constructs induced significant repression of overall promoter activity in comparison with untreated constructs (Fig. 7a).