Next, RNAi-mediated knockdown of glutamine metabolism enzymes revealed that GLS1, GOT1, and ME1 dramatically sensitized MiaPaCa2 and ASPC1 PDA cell lines to ß-lap, relative to non-targeting control (scramble small interfering RNA (siScr)) (Fig. 2g–k). This evidence concerns the gene ME1 and Patent ductus arteriosus.