The results showed that A35 could significantly induce DNA double breakage, and γ-H2AX-positive cells increased to 40%, and the TUNEL results indicated that A35 could induce tumor cell apoptosis and the apoptotic cells comprised up to approximately 70% of total cells (Figure 6C), indicating an identical action mechanism as for the in vitro results. This evidence concerns the gene H2AX and neoplasm.