To verify whether the level of SGK1 expression affected radiosensitivity of liver cancer cell lines, we used lentiviral vectors to produce stably transduced HepG2 and HuH-7 cell lines expressing either SGK1 specific ShRNA (ShSGK1 cells) or wild type SGK1 (p-Hiv-EGFP-SGK1 cells) to induce SGK1 silencing or over expression, respectively. Here, SGK1 is linked to liver cancer.