TP53 and breast cancer: As shown in Figure 1B, a significant inhibition of NFAT1 expression by JapA was observed in MCF-7 (p53 wild-type), MCF-7/p53−/− (p53 knockdown), MDA-MB-231 (p53 mutant), and MDA-MB-468 (p53 mutant) human breast cancer cell lines.