VEGFC and gastric cancer: To determine whether expression of the three miRNAs or VEGF-C correlates with MVD or LVD in human gastric cancers, double immunohistochemical staining for CD34/D2-40 was done to quantitatively evaluate the number of blood vessels (CD34 positive) or lymph vessels (D2-40 positive) in gastric cancer samples (Figure 1D).