The main target genes of CRL4B/PRC2 complex are p16 and PTEN, and CUL4B silencing results in increased expression of p16 and PTEN at both the transcriptional level and the protein level in KYSE410, HeLa, HEK293, MCF-7 (human breast adenocarcinoma cell line), and U2OS (human osteosarcoma cell line). Here, PTEN is linked to osteosarcoma.