LC3 colocalized with LAMP1, a lysosomal marker used for detection of LC3+/LAMP1+ autolysosome formation [26], in pancreatic tumor tissues, and SQSTM1/p62, an autophagy marker that is degraded during autophagy [26], was weakly stained in cells expressing LC3, revealing the activation of autophagy in pancreatic cancer cells (Additional file 1: Figure S1A). This evidence concerns the gene SQSTM1 and pancreatic neoplasm.