To determine the role of autophagy in pancreatic cancer stemness, we established lentivirus-mediated stable ATG5, ATG7, and BECN1 knockdown cell lines shATG5, shATG7, and shBECN1 derived from PANC-1 cells and analyzed their CSC activity. The gene discussed is BECN1; the disease is familial pancreatic carcinoma.