Finally, in agreement with this observation, we showed MEK activation and subsequent ERK and p90RSK phosphorylation were significantly impaired in mouse ovarian tumours following MAP3K8 depletion (Fig. 4f,g), further indicating that MAP3K8-dependent tumour growth is mediated by MEK/ERK/p90RSK activation in vivo. This evidence concerns the gene RPS6KA1 and ovarian neoplasm.