To assess whether KLF12 expression levels correlates with a functional effect on anoikis, an shRNA hairpin was used to knock down its expression in the three lung cancer cell lines used to generate anoikis-resistant cell lines (A549, H23, H460) as well as the additional lung cancer cell line HOP62 that had higher levels of KLF12 expression. This evidence concerns the gene KLF12 and lung cancer.